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. 2019 Jun 28;11:5799–5811. doi: 10.2147/CMAR.S193805

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© 2019 Ma et al.

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Dynamin 3 (DNM3) overexpression induces apoptosis in colon cancer cells. (A) Cell apoptosis was investigated by Annexin V/PI staining and flow cytometry. (B) and (C) Photomicrographs of double-fluorescent staining with PI (red) and Hoechst 33342 (blue) in HCT116 (B) and HT29 (C) cells. PI-positive cells were counted in approximately 200 cells from three random microscopic fields for each sample and are expressed as percentages of the total cells. *P<0.05. All experiments were performed in triplicate.