Figure 3.

Western blotting detecting the pathway of APP/p-ERK/c-Myc/MMP-2. (A) MMP-2, p-ERK and c-Myc expression was significantly decreased after APP expression was attenuated; however, there was no change in the expression of MMP-9 or CXCR4. (B) Kasumi-1 cells treated with a p-ERK inhibitor at concentrations of 20 and 40 µM for 24 and 48 h. The western blotting assay suggested that the expression levels of p-ERK and c-Myc were significantly reduced. (C) The c-Myc inhibitor 10058-F4 was co-cultured with Kasumi-1 cells at a concentration of 50 and 100 µM for 24 and 48 h. The expression of MMP-2 and c-Myc was significantly decreased, but there was no change in p-ERK expression. APP, amyloid precursor protein; CXCR4, C-X-C motif chemokine receptor 4; MMP, matrix metalloproteinase; p-ERK, phosphorylated extracellular-signal-regulated kinase.