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. 2019 Jun 14;18(2):2132–2139. doi: 10.3892/ol.2019.10479

Figure 2.

Figure 2.

Overexpression of NOX5 enhances cisplatin resistance in cancer cells and is associated with increased levels of p-Akt. (A) In vitro toxicity assay of cisplatin treatment in HONE1 cells transfected with pcDNA3.1-NOX5 or pcDNA3.1 empty vector. NOX5-overexpressed HONE1 cells demonstrated increased cell viability at every concentration of cisplatin treatment compared with HONE1 cells transfected with pcDNA3.1. *P<0.05, **P<0.01 vs. control-pcDNA. (B) NOX5 increased the level of p-Akt in HONE1 cells. The protein levels of NOX5, Akt and p-Akt were assessed by western blot analysis 48 h post-transfection. *P<0.05. Data are presented as the mean ± standard deviation, n=3. NOX5, NADPH oxidase 5; p-Akt, phosphorylated Akt; NOX5-OX, HONE1 cells transfected with pcDNA3.1-NOX5.