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. 2019 Jun 18;18(2):2050–2058. doi: 10.3892/ol.2019.10487

Figure 3.

Figure 3.

Long non-coding RNA BLACAT2 accelerates the migration and invasion of colorectal cancer cells. (A) Relative expression levels of BLACAT2 in DLD1 and SW620 cells transfected with shControl, shBLACAT2#1 and shBLACAT2#2 detected by RT-qPCR assay. (B) Relative expression levels of BLACAT2 in DLD1 and SW620 cells transfected with empty vector or BLACAT2 overexpression plasmid detected by RT-qPCR assay. (C) Migration of DLD1 and SW620 cells with BLACAT2 deficiency measured by Transwell assay. Scale bars, 50 µm. (D) Migration of DLD1 and SW620 cells with BLACAT2 overexpression measured by Transwell assay. Scale bars, 50 µm. (E) Invasive capacity of DLD1 and SW620 cells with BLACAT2 deficiency measured by Matrigel assay. Scale bars, 50 µm. (F) Invasive capacity of DLD1 and SW620 cells with BLACAT2 overexpression measured with a Matrigel assay. Scale bars, 50 µm. *P<0.05. BLACAT2, bladder cancer-associated transcript 2; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; sh, short hairpin RNA.