Figure S1. The N2a NM-HAagg bulk population propagates SDS-resistant NM-HA prions.
(A) The presence of SDS-resistant NM-HA polymers in the N2a NM-HAagg bulk population was determined by a filter trap assay (top panel). SDS–PAGE and Western blot analysis were performed as loading control (bottom panel). NM-HA was detected using mAb anti-HA, and actin was detected using mAb anti-actin. (B) SDD–AGE analysis of cell lysates from wild-type N2a cells, N2a cells expressing soluble NM-HA, and the NM-HAagg bulk population. NM-HA was detected using mAb anti-HA. (C) Quantitative analysis of cells bearing aggregates in the N2a NM-HAagg bulk population. Transduced cells were plated in 96-well plates, and six wells were analyzed by automated confocal microscopy. Shown is the number of cells with aggregates within the cell population expressing the transgene. Bars represent mean values ± SD.