Figure 3.

(a) Representative hematoxylin and eosin-stained cryosection from the diaphragm, showing excessive variability in myofiber size, moderate endomysial fibrosis, occasional myofibers showing internal nuclei and multifocal areas of mild adipose tissue infiltration. (b) Representative staining of the vastus lateralis muscle showing type 1 fiber predominance using monoclonal antibodies against slow myosin (type 1 fibers, brown stain) and fast myosin (type 2 fibers, pink stain). Bar in (b) = 50 µm for (a) and (b)