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. 2019 Jun 19;2019:7641767. doi: 10.1155/2019/7641767

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Copyright © 2019 Heidrun Steinle et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Schematic representation of RNA constructs and quality control of DNA templates and synthesized RNA. The srRNA and synthetic modified mRNAs were synthesized using in vitro transcription (IVT). (a) Agarose gel electrophoresis showed the expected lengths for mRNAs encoding the reprogramming factors (Klf4: 1.6 kb; cMyc: 1.5 kb; Oct4: 1.3 kb; Sox2: 1.1 kb; and Lin28: 0.8 kb) and eGFP (0.9 kb). (b) The srRNA contains encoding sequences for the nonstructural proteins (nsP1-4); the reprogramming factors Oct4, Klf4, Sox2, and cMyc; and GFP. Agarose gel electrophoresis showed the expected length (17.7 kb) of linearized DNA and srRNA.