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. 2019 Jun 19;2019:7641767. doi: 10.1155/2019/7641767

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Copyright © 2019 Heidrun Steinle et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

RNA-based iPSC reprogramming of fibroblasts: srRNA vs. mRNA. (a) Emerging of iPSCs over time and detection of GFP-expressing cells during the reprogramming period. Phase contrast and fluorescence microscopic pictures are shown. (b) Live-antibody staining of obtained iPSC colonies. The iPSCs obtained after 20 days of reprogramming by mRNAs were positively stained with DL550 SSEA-4 and DL488 TRA-1-60 antibodies. iPSCs obtained after 12 days of reprogramming by srRNA were positive for SSEA-4 and showed only partial GFP expression within the iPSC colony. Scale bars represent 100 μm.