Figure 6.
Analysis of teratoma formation after the application of mRNA- or srRNA-iPSCs onto the chicken embryo chorioallantoic membrane (CAM). (a) Schematic representation of the teratoma formation analysis on CAM. On day 7 of incubation, 2 × 106 iPSCs were applied into the inner area of a silicone ring placed on CAM. (b) After 10 days (day 17), teratoma were formed on the CAM (indicated by arrows), excised, and embedded in paraffin. (c) Representative microscopic pictures of H&E-stained teratoma sections show the in vivo differentiation of iPSCs into cells of all three germ layers: mesoderm (i: striated muscle fibers, ii: adipocyte tissue), endoderm (glandular epithelium), and ectoderm (squamous epithelium).