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. 2019 Jun 19;2019:7641767. doi: 10.1155/2019/7641767

Table 1.

List of primer sequences used for qRT-PCR analysis.

Gene Forward primer (5′ → 3′) Reverse primer (5′ → 3′)
Pluripotency marker
GAPDH TCAACAGCGACACCCACTCC TGAGGTCCACCACCCTGTTG
Oct4 [3] AGCGAACCAGTATCGAGAAC TTACAGAACCACACTCGGAC
Sox2 [3] AGCTACAGCATGATGCAGGA GGTCATGGAGTTGTACTGCA
Nanog [3] TGAACCTCAGCTACAAACAG TGGTGGTAGGAAGAGTAAAG
Lin28 CTTCTTCTCCGAACCAACC CAGCCACCTGCAAACTG
E-cadherin TATACCCTGGTGGTTCAAGC CACCTGACCCTTGTACGTG
Klf4 [3] TCTCAAGGCACACCTGCGAA TAGTGCCTGGTCAGTTCATC
cMyc [3] ACTCTGAGGAGGAACAAGAA TGGAGACGTGGCACCTCTT
srRNA-specific marker
nsP2 TCCACAAAAGCATCTCTCGCCG TTTGCAACTGCTTCACCCACCC
nsP4 TTTTCAAGCCCCAAGGTCGCAG TGTTCTGGATCGCTGAAGGCAC

GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Oct4: octamer binding transcription factor 4; Sox2: sex-determining region Y-box 2; E-cadherin: epithelial cadherin; Klf4: Krüppel-like factor 4; cMyc: cellular myelocytomatosis; nsP: nonstructural protein.