Table 1.
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
---|---|---|
Pluripotency marker | ||
GAPDH | TCAACAGCGACACCCACTCC | TGAGGTCCACCACCCTGTTG |
Oct4 [3] | AGCGAACCAGTATCGAGAAC | TTACAGAACCACACTCGGAC |
Sox2 [3] | AGCTACAGCATGATGCAGGA | GGTCATGGAGTTGTACTGCA |
Nanog [3] | TGAACCTCAGCTACAAACAG | TGGTGGTAGGAAGAGTAAAG |
Lin28 | CTTCTTCTCCGAACCAACC | CAGCCACCTGCAAACTG |
E-cadherin | TATACCCTGGTGGTTCAAGC | CACCTGACCCTTGTACGTG |
Klf4 [3] | TCTCAAGGCACACCTGCGAA | TAGTGCCTGGTCAGTTCATC |
cMyc [3] | ACTCTGAGGAGGAACAAGAA | TGGAGACGTGGCACCTCTT |
srRNA-specific marker | ||
nsP2 | TCCACAAAAGCATCTCTCGCCG | TTTGCAACTGCTTCACCCACCC |
nsP4 | TTTTCAAGCCCCAAGGTCGCAG | TGTTCTGGATCGCTGAAGGCAC |
GAPDH: glyceraldehyde-3-phosphate dehydrogenase; Oct4: octamer binding transcription factor 4; Sox2: sex-determining region Y-box 2; E-cadherin: epithelial cadherin; Klf4: Krüppel-like factor 4; cMyc: cellular myelocytomatosis; nsP: nonstructural protein.