Table 1.
Gene | Primer sequence (5’→3’) | Product (bp) |
---|---|---|
SREBP1 | Forward 5’-ACACAGCAACCAGAAACTCAAG-3’ | 153 |
Reverse 5’-AGTGTGTCCTCCACCTCAGTCT-3’ | ||
ACLY | Forward 5’-GACCTATGACTATGCCAAGACTAT-3’ | 88 |
Reverse 5’-GATGCTGCCTCCAATGATGA-3’ | ||
ACACA | Forward 5’-AATAGCGTCTCTAACTTCCTTCAC-3’ | 200 |
Reverse 5’-CCGTCACTCAGCCGATGTA-3’ | ||
FASN | Forward 5’-GGACATGGCTTAGAAGTGGAA-3’ | 164 |
Reverse 5’-TTGGTGTTGCTGGTGAGTG-3’ | ||
SCD | Forward 5’-GCGATATGCTGTGGTGCTTA-3’ | 153 |
Reverse 5’-GAGTGGTGGTAGTTGTGGAAG-3’ | ||
β-actin | Forward 5’-TCGTGCGTGACATTAAGGAG-3’ | 176 |
Reverse 5’-GAAGGAAGGCTGGAAGAGTG-3’ |
SREBP1, sterol regulatory element binding protein 1; ACACA, acetyl-CoA carboxylase alpha; ACLY, ATP citrate lyase; FASN, fatty acid synthase; SCD, stearoyl-coenzyme A desaturase.