Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 3;39(27):5269–5283. doi: 10.1523/JNEUROSCI.1833-18.2019

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2019 Mazaud et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Figure 1. — Effect of miRNA expression in adult Drosophila glia. All aging in this figure was performed at 29°C. A, Examples of short-lived (UAS-miR-9c and UAS-miR-1) and long-lived (UAS-miR-100) miRNA lines overexpressed in adult glial cells compared with the screen median lifespan. Each lifespan was done with 60 flies (30 females and 30 males; see Materials and Methods). Log–rank (Mantel–Cox) test p < 0.000000000001 for all. For a full screen report, see Figure 1-1. B, Summary of the miRNA screen. Each line is compared with the median lifespan of the library (256 lines from 133 miRNAs). C, Comparison of the median from 6 w¹¹¹⁸; ubi-Gal80^ts/+; repoGal4/+ lifespan with the median lifespan of the screen w¹¹¹⁸; (ubi-Gal80^ts; repoGal4)/ UAS-miR-X. Log–rank (Mantel–Cox) test p < 0.0001 (D) Lifespan of female flies expressing UAS-miR-1, from an autosomal insertion (7091) and from an insertion on the X chromosome (7090) compared with female median w¹¹¹⁸ controls. Both display a dramatic reduction in lifespan. Log–rank (Mantel–Cox) test p < 0.0001 for both. E, Western blot analysis of Repo protein level upon miR-1 overexpression in adult glia. There is a strong reduction of Repo protein compared with control, quantified on the right panel (Mann–Whitney, n = 3, p = 0.049). The genotypes were w¹¹¹⁸; ubi-Gal80^ts/+; repoGal4/+ and w¹¹¹⁸; ubi-Gal80^ts/+; repoGal4/UAS-miR-1 (7091). F, Rescue of UAS-miR-1 short lifespan by coexpression of UAS-repo-myc (log–rank Mantel–Cox test p < 0.000000000001), which by itself displays instead a mildly decreased lifespan compared with controls. Conversely, the coexpression of a mutated dominant-negative repo transgene, UAS-repoΔHD-myc, enhanced the shortening of lifespan due to miR-1 overexpression (log–rank Mantel–Cox test p < 0.0001), ruling out any weakening of the UAS-miR-1 effect due to a second UAS-based transgene, whereas it had no effect of its own when expressed alone. The driver used was repoGal4, ubi-Gal80^ts. Between 80 and 180 female flies were assessed. *p < 0.05, ***p < 0.001, ****p < 0.0001.