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. 2019 Jul 3;39(27):5269–5283. doi: 10.1523/JNEUROSCI.1833-18.2019

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Copyright © 2019 Mazaud et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 8. — Effect of Gs2 and Gat on endogenous motor activity and recovery from seizures. A, Constant tracking of the flies allows the analysis of the endogenously generated motor activity represented here by the three functions active speed, mean bout length, and interbout interval (see Materials and Methods). The flies and data used are the same as in Figure 7C. Although the active speed does not seem to be strongly affected, the flies expressing only UAS-repo beside UAS-repo^IR 1 have a significant reduction in mean bout length after 17 d and a strong increase of the interbout interval also after 17 d. These changes are not present when either UAS-Gat or UAS-Gs2 is coexpressed. For simplicity, statistical comparisons represented are between UAS-repo vs UAS-Gat + UAS-repo and UAS-repo vs UAS-Gs2 + UAS-repo only (two-way ANOVA, Tukey's multiple-comparisons test p = 0.0004, p = 0.0002, p = 0.0077, p = 0.0300, and p < 0.0001, respectively). B, Time to recovery analysis of epileptic-like features at 3 and 11 d at 25°C. Ten flies were analyzed for each genotype at each time point, repeated five times independently, and averaged. UAS-repo and UAS-Gat co-overexpression significantly rescued the seizure phenotype due to repo knock-down. Black asterisks indicate significance in genotype/time interactions compared with UAS-repo alone (two-way ANOVA, Dunnett's multiple-comparisons test p = 0.0029). Colored asterisks indicate significance between the different genotypes and UAS-repo alone at a specific time point (two-way ANOVA, Dunnett's multiple-comparisons test p = 0.0545, p = 0.0248, and p < 0.0001, respectively).