FIGURE 6.

Chemically induced aggregation of denatured lysozyme (35 μM) by DTT (20 mM) in the presence and absence of conjugated B1NTR-AuNPs. (A) Chemically induced aggregation of 35 μM Lys (black), Lys (35 μM) in presence of B1NTR (15 μM, green), and B1NTR only (15 μM, blue) with DTT (20 mM) at 37°C. (B) Aggregation of Lys in the presence of DTT (20 mM) at 37°C in the presence of conjugated B1NTR-AuNPs using 10 nm AuNPs (OD 10) at dilutions 1:100 and 1:500 (final concentration), and PEG-AuNP (1:8 dilution, 10 nm AuNP). All dilutions are calculated relative to the initial concentration of OD 10 for each 10 nm AuNP sample. All dilutions performed in 1× PBS, pH 7.4 (C) Chemically-induced aggregation of Lys (35 μM) with DTT (20 mM) at 37°C in the presence of B1NTR-AuNPs using 5 nm AuNPs (OD 2) at final dilutions of 1:20 (purple) and 1:100 (orange) relative to starting OD 2 for each AuNP sample. Each curve is the average of ≥3 independent trials