Figure 2.

Glycan residues are crucial in SLP-8348 immunogenicity. Cells from inguinal lymph nodes were obtained from BALB/c mice subcutaneously injected with one dose (10 μg/mouse) of SLP-8348 or PBS in combination or not with incomplete Freund's adjuvant. IFN-γ levels on supernatants and proliferation index of CD4⁺ T cells (calculated as the ratio between the percentage of CFSE ^low CD4⁺ cells and CFSE^low CD4⁺ cells from PBS+IFA-treated mice) were measured after stimulation with SLP-8348 (10 μg/ml) for 5 days (A). The same experiment was performed using SLP-8348, oxidized SLP-8348 (SLPOx-8348), and oxidation negative control (SLPB-8348) in the absence of adjuvant. IFN-γ levels in supernatants and proliferation index of CD4⁺ T cells (calculated as the ratio between the percentage of CFSE ^low CD4⁺ cells and CFSE^low CD4⁺ cells from PBS-treated mice) were measured after stimulation with SLP-8348 (10 μg/ml) for 5 days (B). A representative figure of three independent experiments is shown (±SD, indicated by error bars). Asterisks indicate statistically significant differences by one-way ANOVA with Tukey's post hoc test (^*p < 0.05).