Skip to main content
. 2019 Jun 26;10:1422. doi: 10.3389/fimmu.2019.01422

Figure 5.

Figure 5

SLP-8348-induced activation of BMDCs is Mincle-dependent. BMDCs from C57BL/6 mice were stimulated with SLP-8348, LPS or the combination of both for 24 h. Culture supernatants and cells were collected and analyzed for the levels of IL-6 and TNF-α and for the cell surface expression of CD40 and CD80, respectively (A,B). BMDCs from Mincle−/− mice, CARD9−/− and SignR3−/− mice were stimulated with SLP-8348, LPS or the combination of both for 24 h. Culture supernatants were collected and analyzed by ELISA for IL-6 and TNF-α (C). Cells were collected and cell surface expression of CD40 and CD86 on CD11c+ cells were analyzed by Flow cytometry (D) A representative figure of three independent experiments is shown (±SD, indicated by error bars). Asterisks indicate statistically significant differences by two-way ANOVA with Tukey's post hoc test (*p < 0.05); n.s: not significant; KO: knock-out.