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. 2019 Jun 26;10:1422. doi: 10.3389/fimmu.2019.01422

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Copyright © 2019 Malamud, Carasi, Assandri, Freire, Lepenies and Serradell.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Interaction between SLP-8348 and Mincle favors the adjuvanticity of SLP-8348. BMDCs from C57BL/6 mice were stimulated with SLP-8348, OVA or the combination of both for 24 h. Cells were collected and exposed to CFSE-labeled CD4⁺ T cells from OT-II mice. Proliferation of CD4⁺ T cells (evaluated on CFSE-stained CD4⁺T cells) and IFN-γ levels in supernatants were measured after 5 days (A). The same experiment was performed using BMDCs from CLR-deficient mice. Proliferation index of CD4⁺ T cells within each experimental group was calculated as the ratio between the percentage of CFSE ^low CD4⁺ cells and CFSE^low CD4⁺ cells from OVA-treated BMDCs (B) A representative figure of three independent experiments is shown (±SD, indicated by error bars). Asterisks indicate statistically significant differences by two-way ANOVA with Tukey's post hoc test (^*p < 0.05).