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Expression of the three reporters in recombinant E. coli Top10. The strains harboring the lac, ara, and pbr promoter reporter systems presented β-galactosidase (A,B) or mCherry signal (C) when cultured in the presence of 0.06 mM IPTG, 0.002% arabinose plus 0.1 mM IPTG, and 20 mM Pb(II) plus 0.1 mM IPTG, respectively. Extra substrate, 0.4% X-gal or 0.4% ONPG, was added to LB plate for the detection of β-galactosidase activity.
