TABLE 1.
Strains/plasmids | Genotypes or description | Source |
E. coli Top10 | F–, Φ80lacZΔM15, ΔlacX74, recA1 | Invitrogen |
E. coli DH5α | F–, Φ80lacZΔM15 Δ(lacZYA-argF), U169, recA1 | Invitrogen |
pBR322 | AmpR, TetR, ori pMB1, commonly used E. coli cloning vector | Novagen |
pUCm-T | TA cloning | Sangon |
pBAD | AmpR, ori pBR322, containing araBAD promoter | Thermo Fisher Scientific |
pT-RFP | pUCm-T carrying mCherry | Hui et al.,2018c |
pPlac-lacZα | AmpR, ori pMB1, pBR322 derivative with lacZα peptide expressing under lac promoter | This study |
pPlac-lacZ | pBR322 derivative with β-galactosidase expressing under lac promoter | This study |
pPlac-RFP | pBR322 derivative with mCherry protein expressing under lac promoter | This study |
pPara-lacZα | AmpR, ori pMB1, pBR322 derivative with lacZα peptide expressing under araBAD promoter | This study |
pPara-lacZ | pBR322 derivative with β-galactosidase expressing under araBAD promoter | This study |
pPara-RFP | pBR322 derivative with mCherry protein expressing under araBAD promoter | This study |
pT-Ppbr | pUCm-T carrying pbrR and divergent pbr promoter region | This study |
pPpbr-RFP | pPlac-RFP derivative with pbrR and pbr promoter preceding mCherry, a single RFP reporter system | This study |
pPpbr-lacZα | pPlac-lacZα derivative with pbrR and pbr promoter preceding lacZα, a single lacZα reporter system | This study |
pPpbr-lacZ | pPlac-lacZα derivative with pbrR and pbr promoter preceding lacZ, a single lacZ reporter system | This study |
The cloning/expression regions of recombinant plasmids used in this study are shown in Supplementary Figure S1.