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. 2019 Jun 26;10:1454. doi: 10.3389/fmicb.2019.01454

TABLE 1.

Bacterial strains and plasmids used in this study.

Strains/plasmids Genotypes or description Source
E. coli Top10 F, Φ80lacZΔM15, ΔlacX74, recA1 Invitrogen
E. coli DH5α F, Φ80lacZΔM15 Δ(lacZYA-argF), U169, recA1 Invitrogen
pBR322 AmpR, TetR, ori pMB1, commonly used E. coli cloning vector Novagen
pUCm-T TA cloning Sangon
pBAD AmpR, ori pBR322, containing araBAD promoter Thermo Fisher Scientific
pT-RFP pUCm-T carrying mCherry Hui et al.,2018c
pPlac-lacZα AmpR, ori pMB1, pBR322 derivative with lacZα peptide expressing under lac promoter This study
pPlac-lacZ pBR322 derivative with β-galactosidase expressing under lac promoter This study
pPlac-RFP pBR322 derivative with mCherry protein expressing under lac promoter This study
pPara-lacZα AmpR, ori pMB1, pBR322 derivative with lacZα peptide expressing under araBAD promoter This study
pPara-lacZ pBR322 derivative with β-galactosidase expressing under araBAD promoter This study
pPara-RFP pBR322 derivative with mCherry protein expressing under araBAD promoter This study
pT-Ppbr pUCm-T carrying pbrR and divergent pbr promoter region This study
pPpbr-RFP pPlac-RFP derivative with pbrR and pbr promoter preceding mCherry, a single RFP reporter system This study
pPpbr-lacZα pPlac-lacZα derivative with pbrR and pbr promoter preceding lacZα, a single lacZα reporter system This study
pPpbr-lacZ pPlac-lacZα derivative with pbrR and pbr promoter preceding lacZ, a single lacZ reporter system This study

The cloning/expression regions of recombinant plasmids used in this study are shown in Supplementary Figure S1.