Table 3.
Gas exchange rate and chlorophyll fluorescence measured with Li-Cor of 5-month-old guayule control and AOS-transgenic lines.
| Genotypes | Pn (μmol CO2 m−2 s−1) | g (μmol CO2 m−2 s−1) | € (mol H2O m−2 s−1) | ΦPSII | ETR (μmol m−2 s−1) | NPQ |
|---|---|---|---|---|---|---|
| RT | ||||||
| WT | 5.75 ± 0.8 | 0.093 ± 0.03 | 2.33 ± 0.6 | 0.146 ± 0.015 | 115.73 ± 11.3 | 1.97 ± 0.2 |
| pND6.10 | 6.29 ± 0.7 | 0.110 ± 0.03 | 2.74 ± 0.6 | 0.141 ± 0.015 | 111.1 ± 11.6 | 1.74 ± 0.1 |
| pND6.12 | 6.20 ± 0.8 | 0.109 ± 0.02 | 2.66 ± 0.5 | 0.145 ± 0.015 | 114.4 ± 12.1 | 1.90 ± 0.2 |
| AOSi 7.2 | 8.56 ± 0.6∗∗∗ | 0.147 ± 0.01∗∗∗ | 3.41 ± 0.3∗∗∗ | 0.196 ± 0.027∗ | 165.2 ± 10.7∗∗∗ | 1.33 ± 0.2∗∗∗ |
| AOSi 8.1 | 8.40 ± 0.6∗∗∗ | 0.155 ± 0.02∗∗∗ | 3.67 ± 0.4∗∗∗ | 0.180 ± 0.008∗ | 141.4 ± 6.2∗∗ | 1.27 ± 0.2∗∗∗ |
| AOSi 9.16 | 7.96 ± 0.5∗∗∗ | 0.162 ± 0.03∗∗∗ | 3.57 ± 0.5∗∗∗ | 0.186 ± 0.018∗ | 134.4 ± 3.3∗∗ | 1.19 ± 0.3∗∗∗ |
| AOSoe 4.1 | 5.62 ± 0.9 | 0.110 ± 0.04 | 2.59 ± 0.7 | 0.133 ± 0.008 | 104.7 ± 6.7 | 1.92 ± 0.3 |
| AOSoe 5.1 | 5.70 ± 0.8 | 0.110 ± 0.04 | 2.60 ± 0.7 | 0.133 ± 0.008 | 104.7 ± 6.7 | 1.95 ± 0.3 |
| AOSoe 7.1 | 5.87 ± 0.9 | 0.113 ± 0.04 | 2.65 ± 0.7 | 0.137 ± 0.008 | 107.7 ± 6.3 | 2.07 ± 0.4 |
| Cold | ||||||
| WT | 2.23 ± 0.5 | 0.054 ± 0.02 | 1.31 ± 0.4 | 0.070 ± 0.007 | 55.4 ± 5.4 | 1.58 ± 0.2 |
| pND6.10 | 2.04 ± 0.4 | 0.057 ± 0.02 | 1.55 ± 0.3 | 0.064 ± 0.006 | 50.7 ± 4.9 | 1.56 ± 0.2 |
| pND6.12 | 2.28 ± 0.4 | 0.065 ± 0.03 | 1.67 ± 0.7 | 0.066 ± 0.003 | 51.6 ± 2.7 | 1.51 ± 0.3 |
| AOSi 7.2 | 4.14 ± 0.4∗∗∗ | 0.104 ± 0.02∗∗∗ | 2.54 ± 0.4∗∗∗ | 0.104 ± 0.011∗∗∗ | 81.0 ± 8.2∗∗∗ | 2.29 ± 0.3∗∗ |
| AOSi 8.1 | 4.15 ± 0.4∗∗∗ | 0.112 ± 0.05∗∗∗ | 2.71 ± 0.8∗∗∗ | 0.102 ± 0.013∗∗∗ | 77.0 ± 4.5∗∗∗ | 2.33 ± 0.2∗∗ |
| AOSi 9.16 | 4.14 ± 0.6∗∗∗ | 0.101 ± 0.03∗∗∗ | 2.60 ± 0.5∗∗∗ | 0.101 ± 0.009∗∗∗ | 79.4 ± 6.9∗∗∗ | 1.95 ± 0.1∗∗ |
| AOSoe 4.1 | 2.27 ± 0.5 | 0.059 ± 0.02 | 1.51 ± 0.3 | 0.065 ± 0.011 | 60.4 ± 5.5 | 1.35 ± 0.3 |
| AOSoe 5.1 | 2.97 ± 0.3 | 0.069 ± 0.01 | 1.81 ± 0.2 | 0.077 ± 0.007 | 58.5 ± 6.3 | 1.25 ± 0.2 |
| AOSoe 7.1 | 2.45 ± 0.6 | 0.063 ± 0.01 | 1.93 ± 0.3 | 0.065 ± 0.010 | 50.1 ± 5.8 | 1.44 ± 0.2 |
Pn, net photosynthetic rate; g, stomatal conductance; E, Transpiration rate; PSII, Efficiency of Photosystem II; ETR, Electron Transport Rate; NPQ, Non-photochemical quenching. Li-Cor readings are the average of three biological replicates ± SD. (∗), (∗∗) and (∗∗∗) indicate significant difference in comparison to WT at p > 0.05, 0.005, and 0.0005, respectively. Data are representative of three independent experiments.