Table 5.
Liquid chromatography-tandem spectrometry hormone analysis of stem and leaf tissue from 8-week-old tissue-cultured guayule control and AOS-transgenic lines.
| Content (ng/gfw) |
|||||||
|---|---|---|---|---|---|---|---|
| Genotypes | Jasmonic Acid | Salicylic Acid | Abscisic Acid | Gibberellin A20 | Gibberellin A1 | Gibberellin A3 | 12-oxo-phytodienoic acid |
| WT | 5.36 + 1.2 | 5.50 + 0.8 | 11.01 + 1.9 | 15.95 + 0.7 | 9.95 + 0.07 | 3.52 + 0.2 | 2.96 + 0.7 |
| pND6.12 | 1.57 + 0.1 | 4.89 + 0.6 | 7.05 + 0.8 | 14.11 + 1.2 | 12.39 + 2.2 | 2.19 + 0.01 | 5.44 + 0.3 |
| pND6.33 | 4.76 + 1.0 | 5.04 + 0.1 | 7.24 + 0.3 | 13.86 + 2.6 | 12.70 + 2.3 | 1.79 + 0.09 | 3.60 + 0.8 |
| pND6.35 | 1.96 + 0.4 | 5.6 + 0.5 | 7.10 + 0.6 | 14.41 + 1.2 | 14.65 + 0.2 | 2.16 + 0.3 | 2.17 + 0.3 |
| AOSi 7.2 | 0.57 + 0.1∗∗ | 9.51 + 0.5∗ | 4.71 + 0.6∗ | 9.63 + 1.2∗ | 5.13 + 0.8∗ | 0.86 + 0.3∗ | 0.76 + 0.2∗ |
| AOSi 9.16 | 0.57 + 0.01∗∗ | 7.65 + 0.2∗ | 2.96 + 0.3∗ | 8.85 + 2.1∗ | 7.81 + 0.2∗∗ | 0.80 + 0.3∗ | 0.39 + 0.07∗ |
| AOSi 12.1 | 0.68 + 0.05∗∗ | 9.65 + 1.1∗ | 3.64 + 0.5∗ | 10.59 + 0.2∗ | 6.75 + 1.1∗ | 1.32 + 0.07∗ | 0.81 + 0.14∗ |
| AOSoe 4.1 | 1.48 + 0.2 | 4.03 + 0.7 | 9.13 + 1.71 | 15.49 + 0.6 | 10.78 + 0.1 | 1.93 + 0.06 | 2.33 + 0.1 |
| AOSoe 4.2 | 3.25 + 0.2 | 4.76 + 0.6 | 13.9 + 1.3 | no data | 14.63 + 2.7 | 1.80 + 0.1 | 2.84 + 0.8 |
| AOSoe 7.1 | 1.41 + 0.3 | 5.50 + 0.02 | 8.84 + 0.3 | 13.64 + 1.2 | 13.96 + 0.01 | 1.84 + 0.1 | 2.68 + 0.7 |
Values represent the average ± standard deviation of three biological replicates. (∗) and (∗∗) indicates significant difference in comparison to G7-11 at p > 0.05 and 0.005, respectively.