Table 3.
Primer sequences and restriction enzymes used for restriction analysis.
| Gene | SNP position GRCh37/hg19 | SNP ID | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) | Tm (°C) | Ref allele SNP allele | Restriction enzyme |
|---|---|---|---|---|---|---|---|
| ADAMTS3 | 4:73414590 | – | TCACCCCACAGATTTACCATTA | GGGCTTTAGTCGCAGATGAA | 60 | A:139+46+20 G:159+46 | MseI |
| ANKRD55 | 5:55407449 | rs77017041 | GGTGATGATGTCATTGACTGCTG | TACTCACATATCATCCCTGCTCTTT | 60 | A:201+21 G:222 | Bpu10I |
| CD86 | 3:121774281 | rs11575853 | CTGCTGTAACAGGGACTAGCTCA | AGGAACTAAGTGAAGGACACACATC | 60 | A:176+23 G:199 | Hpy188I |
| EVI5 | 1:92979432 | rs41286809 | ACACATAGAAGGCACTCAAAAATTAG | CTATAAAATCTTCATCGGAGGACTG | 60 | C:250+25 T: 275 | Bsr I |
| GC | 4:72669661 | rs76781122 | CCACTAATGCCAGCCAATCT | TGCTTTGCACAGAAATCCTC | 60 | G:361+48 T:266+95+48 | ApoI |
| IL2RA | 10:6054765 | rs12722600 | AACAGAAGTCATGAAGCCCACGT | AGTGGTTTTGCCCTTCCTC | 60 | G:219+21 A:240 | PmlI |
| MALT1 | 18:56367823 | rs74847855 | CACTTTCAAAGCTTCATACTGAAATC | AAGACAAAACACATGGATCAAATCT | 60 | A:248+179 G:427 | Hpy188III |
| MMEL1 | 1:2530169 | rs147248515 | CACTAAAGCTTAACCCCTCATGTC | TATCCTCTGTCAAAATCAAGCTGGT | 60 | G:221+27 T:248 | BanI |
| TET2 | 4:106156163 | rs61744960 | CTGATGATGCTGATAATGCCAGT | GTAAGCACCATTCATTTCATTTTGT | 60 | G:134+75+39 A:134+114 | NlaIV |
| TOP3A | 17:18217958 | rs2230153 | TCGCCTTCATCTCGATTCTT | TGAGCCTCATCTCTGGCTTC | 55 | G:190+134+18 A:190+152 | EaeI |
| TRAF3 | 14:103371923 | rs138943371 | ATGTGTGCCAGGGTCTACCT | TCTTGAAGCTGCTGCTGTTG | 63 | C:146+62 T:208 | AvaII |
| TYK2 | 19:10472452 | rs12720355 | GGACCCTAGTCACCATGAGAT | GTCTCGTAGAAGGCCTGTGG | 60 | C:197+18 T:215 | MboI |
| WWOX | 16:78458807 | rs7201683 | AAAGAATTTCTCATTCCCGAAG | CACCCACATGTCTCAAGCAG | 60 | C:444 G:259+185 | RsaI |
To verify the presence of the selected mutations, PCR products were digested with restriction enzymes. Nucleotides in bold and underlined are those modified to create specific restriction sites.