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. 2019 Jun 26;10:1471. doi: 10.3389/fimmu.2019.01471

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Copyright © 2019 Tang, Yu, Li, Zhang, Xie and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative FACS results of Tregs from one experiment. (A) C57BL/6 group; (B) SEA group; (C) Lepr^db/db group; (D) Lepr^db/db+ SEA group. Upper panels, the numbers denote the frequency of CD4⁺ T cells in splenocytes. Lower panels, the right upper quadrant indicates the frequency of CD25⁺Foxp3⁺ T cells from CD4⁺ lymphocytes. (E) Upper panel, the numbers denote the frequency of lymphocytes. Lower panel, APC-conjugated rat IgG2α served as isotype control.