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. 2013 Jun 14;94(6):1103–1112. doi: 10.1189/jlb.0213064

Figure 4.

Figure 4

Cdkn2a is critical for IL‐4‐induced IL‐10 production. (A) Splenocytes from B6 WT mice were transfected with FAM‐conjugated siRNAs and cultured under the Con A + IL‐2 + IL‐4 condition for 48 h; cells transfected with siRNA (FAM+ cells) were sorted and lysed for real‐time PCR to detect the expression level of the indicated gene. The cDNA was also used to analyze the mRNA levels of IL‐10 by real‐time PCR (B). Data are shown as mean + sem of three independent experiments performed; **P < 0.01; ***P < 0.001. (C) Splenocytes from B6 WT mice were transfected with Cdkn2a or blimp1 siRNA and cultured with Con A + IL‐2 + IL‐4 for 48 h; FAM+ cells were sorted for real‐time PCR analysis of blimp1 and Cdkn2a, respectively. Data are representative of at least three independent experiments (mean+sem). No statistical difference was observed. Pparg, Peroxisome proliferator‐activated receptor γ; Elk3, a member of ETS oncogene family; Fli1, Friend leukemia integration 1 transcription factor.