Figure 3.

Sialyltransferase deficiency affects neutrophil trafficking to the peritoneum but not the lung. WT and ST3Gal4^−/− (A, C) or WT and ST6Gal1^−/− (2 × 10⁶) (B, D) neutrophils (2 × 10⁶) were injected in a 1:1 ratio into WT‐recipient animals. Animals were given intratracheal NTHI (C, D) or intraperitoneal thioglycollate (A, B) 1 h before neutrophil injection. Open bars: injected ratio of sialyltransferase KO neutrophils to WT neutrophils. Solid bars: ratio of cells recovered from the peritoneum or BALF. In general, sialyltransferase deficiency did not affect neutrophil trafficking to the lung. ST3Gal4 deficiency reduced neutrophil migration to peritoneum (n = 8 mice for each subgroup). *P < 0.01 vs. injection ratio.