Figure 5.

Blocking the α and β subunits of the β2‐integrins normalizes the excess ICAM‐1‐mediated recruitment of the Fut4^−/−Fut7^−/− neutrophils. Colony‐forming units of NTHI (1 × 10⁶) were instilled intratracheally into WT and Fut4^−/−Fut7^−/− mice immediately after the injection of 50 μg of anti‐CD11a mAb M17/4, anti‐CD11b mAb M1/70, or anti‐CD18 mAb M18/2. BALF was collected, and total cell counts were determined as described in Fig. 4A. Total neutrophil counts were determined as in Fig. 4B. (C–F) H&E‐stained lung sections of WT or Fut4^−/−Fut7^−/− animals treated with NTHI and no mAb (C), CD11a (D), CD11b (E), or CD18 (F) showed a normalization of the inflammation and inflammatory cell numbers between WT and Fut4^−/−Fut7^−/− lungs upon blocking with any of the β₂‐integrin mAbs (n = 8 mice for each subgroup). Magnification, ×40 and ×200 (inset). Scale bar, 100 μm. ***P < 0.001 vs. WT of each subgroup. ^† P < 0.01 vs. Fut4^−/−Fut7^−/−+NTHI sample.