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. 2010 Jul 13;88(4):687–697. doi: 10.1189/jlb.1109756

Figure 2.

Figure 2

Immunoprecipitation, N‐deglycosylation, and phosphorylation of mouse CD84. (A) The biotinylated cell lysates from CD84 transiently transfected COS‐7 cells were immunoprecipitated (IP) with mCD84.7 mAb and treated with (+) or without (–) N‐glycosylase F (N‐Glyc. F). WB: Sv‐POD, Western blot streptavidin‐POD. Biotinylated thymocyte, splenocyte, and DC (B) lysates were immunoprecipitated with the mCD84.7 mAb or with an isotypic hamster IgG as a control. (C) Phosphorylation of mouse CD84 Y (pY) in immature DCs. Untreated (–) and pervanadate (PV)‐treated (+) cells were biotinylated and lysed and then immunoprecipitated with mCD84.7 mAb. (D) Phosphorylation of mouse CD84 Y in LPS‐stimulated CD84‐RAW‐264.7 cells. Molecular mass in kDa was determined by the migration of a known protein standard.