Figure 5.

CLU causes aggregation of NK cells without significantly altering surface NK receptors. (A) Purified NK cells (7.5×10⁵) were incubated with 5 μg/ml CLU in the presence or absence of IL‐2 (100 U/ml) for 5 days prior to taking photos using an inverted microscope (40× and 100× original magnification). (B) Surface expression of CD27, CD44, CD11c, LFA‐1, I‐Ab, NKG2D, CD69, or ICAM‐1 was monitored by FACS using NK cells cultured with 100 U/ml IL‐2 in the presence or absence of 5 μg/ml CLU. The filled line represents isotype control, thin line IL‐2 alone, and thick line IL‐2 + CLU‐cultured NK cells. The results of this particular experiment represent those from 3 other independent experiments.