Figure 4.

Effect of galectin‐12 knockdown on NB4 cell differentiation. (A) Expression of differentiation maker CD11b under different treatment conditions. Cells were treated with or without doxycycline (dox) in the presence or absence ATRA for 72 h, then incubated with anti–CD11b‐FITC. At least 1 × 10⁴ cells were analyzed in each group by flow cytometry. Histograms represent median fluorescence intensity (MFI) of CD11b expression in each treatment group from 3 independent experiments. (B) Induction of NBT‐reducing activity of NB4 cells under different treatment conditions. After the same treatments as described for Fig. 4A, cells were coincubated with NBT and PMA as described in MATERIALS AND METHODS. Formazan was then dissolved in 2 M KCl and DMSO, and absorbance at 620 nm was measured. *P < 0.05.