Figure 2.

Burn injury predisposes the terminal fate of CMPs. CMPs have tripotency when placed in physiologic conditions; all 3 lineages are produced from CMPs. Characteristics of sorted CMPs before differentiation and after cultured to CMP → MØ, CMP → DC, and CMP → EB via morphology by May‐Grünwald‐Giemsa staining technique are shown and read by light microscopy at 20× original magnification using the EVOS FL Cell Imaging System (A). Flow cytometric inspection, using cell‐surface markers F480^hiERMP20^low (MØs), CD11c ^hiF480^low (DCs), and CD71⁺ Ter119⁺ (EBs) compared with sham (left) and burn (right), is shown (B), and the means ± sem of CMP derivatives are depicted as bar graphs (C). Despite standard ex vivo conditions, burn injury skewed the terminal fate of CMPs away from EB generation (burn: 18.7 ± 1.2%, sham: 34.5 ± 1.7%). DC production was also impaired following burn injury (burn: 43 ± 5%, sham: 60 ± 3%), whereas MØ production was augmented by burn (burn: 65 ± 7%, sham: 38 ± 4.8%). *P < 0.05 vs. Sham.