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. 2015 Oct 1;99(3):483–493. doi: 10.1189/jlb.4A0315-115RR

Figure 8.

Figure 8

Cytokine production profiles of Gr‐1+ and Gr‐1− γδ T cells after lung infection. Cells prepared from BAL, spleen (SPL), or lymph node (LN) from infected mice were incubated for 4 h at 37°C with Brefeldin A to allow for intracellular accumulation of cytokines. Cells were stained for CyTOF analysis to detect the indicated cytokines and RoRγt in gated Gr‐1+ or Gr‐1−, CD3+/TCRγδ + cell populations. The results of 3 (BAL) or 2 (SPL, LN) experiments each are plotted as means ± sem (n = 4 mice per group). *P < 0.05, **P < 0.01, ***P < 0.001 for Gr‐1− vs. Gr‐1+ γδ T cells.