Table 2.
MMC induces excessive chromosomal breaks and radial formation in T‐shFC cells
| Cell type | Treatment | Concentration | Radials (%) | Breaks per cell |
|---|---|---|---|---|
| T‐shNT | Control | 2 | 0.1 | |
| MMC | 60 ng/ml | 4 | 0.18 | |
| MMC | 80 ng/ml | 4 | 0.2 | |
| T‐shFC | Control | 0 | 0 | |
| MMC | 60 ng/ml | 8 | 0.44 | |
| MMC | 80 ng/ml | 10 | 0.46 | |
| T‐shNT | Control | 0 | 0.02 | |
| R848 | 3 µM | 0 | 0.02 | |
| R848 | 30 µM | 0 | 0.12 | |
| T‐shFC | Control | 0 | 0.02 | |
| R848 | 3 µM | 0 | 0.02 | |
| R848 | 30 µM | 0 | 0 |
In response to MMC (48 h), T‐shFC cells acquired more breaks per cell and radial forms than did T‐shNT cells, but R848 treatment (48 h) failed to induce breaks or radials in either cell type.