Table 3.
DNA damage does not induce TNF‐α production by macrophages
| Cell line | Treatment | TNF‐α (pg/ml) | sd |
|---|---|---|---|
| T‐shNT | DMSO | Undetectable | NA |
| T‐shFC | DMSO | Undetectable | NA |
| T‐shNT | R848 (30 μM) | 984 | 147 |
| T‐shFC | R848 (30 μM) | 2349 | 208 |
| T‐shNT | MMC (100 nM) | Undetectable | NA |
| T‐shFC | MMC (100 nM) | Undetectable | NA |
| T‐shNT | MMC (300 nM) | Undetectable | NA |
| T‐shFC | MMC (300 nM) | Undetectable | NA |
| T‐shNT | MMC (1 μM) | Undetectable | NA |
| T‐shFC | MMC (1 μM) | Undetectable | NA |
| T‐shNT | H2O2 (0.00003%) | Undetectable | NA |
| T‐shFC | H2O2 (0.00003%) | Undetectable | NA |
| T‐shNT | H2O2 (0.0003%) | Undetectable | NA |
| T‐shFC | H2O2 (0.0003%) | Undetectable | NA |
| T‐shNT | H2O2 (0.003%) | Undetectable | NA |
| T‐shFC | H2O2 (0.003%) | Undetectable | NA |
| T‐shNT | CPT (100 nM) | Undetectable | NA |
| T‐shFC | CPT (100 nM) | Undetectable | NA |
| T‐shNT | CPT (1 μM) | Undetectable | NA |
| T‐shFC | CPT (1 μM) | Undetectable | NA |
T‐shNT and T‐shFC cells were plated at a concentration of 106 cells/ml and treated with R848 (30 µM), MMC (100 nM, 300 nM, or 1 µM), H2O2 (0.00003%, 0.0003%, or 0.003%), or CPT (100 nM or 1 µM) for 24 h. Mean (TNF‐α) ± sd was derived from 3 biologic replicates for each condition. NA, not available.