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. 2017 Jan 13;102(1):7–17. doi: 10.1189/jlb.3HI0316-152R

Figure 1.

Figure 1

Particle‐induced cell death is independent of both inflammasomes and RIP3. In all cases, PMs were primed with LPS. (A) PMs from WT, NLRP3−/−, ASC−/−, or Casp1−/− stimulated with LLOMe (1 mM), silica (50 μg/ml), MSU (250 μg/ml), or CCs (750 μg/ml). (B, left panel) PMs from WT or RIP3−/− mice stimulated with silica (50 μg/ml) or AT406 (10 μM). (B, right panel) PMs from WT or NLRP3−/− mice were stimulated with silica (40 μg/ml), nigericin (2 μM), or AT406 (7.5 μM). Error bars represent (A) WT (LLOMe n = 6, silica n = 6, MSU n = 4, CCs n = 4), NLRP3−/− (LLOMe n = 3, silica n = 4, MSU n = 3, CCs n = 3), ASC−/− (LLOMe n = 4, silica n = 3, MSU n = 3, CCs n = 3), Casp1−/− (LLOMe n = 5, silica n = 3, MSU n = 3, CC n = 3); or (B, left panel) n = 4, (B, right panel) n = 3. Statistical analysis was performed by 2‐way ANOVA and Dunnett's multiple comparisons test (A), or 2‐tailed Student's t test (B). *P < 0.05, **P < 0.01, ***P < 0.001 ****P < 0.0001.