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. 2017 Jan 13;102(1):7–17. doi: 10.1189/jlb.3HI0316-152R

Figure 2.

Figure 2

Cathepsin inhibitors and not‐caspase inhibitors suppress both particle‐induced cell death and IL‐1β secretion. In all cases, PMs were primed with LPS. (A) PMs from WT, ASC−/−, Casp‐1−/− mice treated with K777 (30 μM) before stimulation with silica (80 μg/ml) or dAdT (1 μg/ml). (B) PMs treated with media control (black bars, [−]), caspase‐1 inhibitors (gray bars, YVAD [20 μM], ZVAD [10 μM]), or cathepsin inhibitors (white bars, K777 [20 μM], Ca074Me [20 μM]) before stimulation with silica (50 μg/ml), LLOMe (1 mM), or CCs (750 μg/ml). Error bars represent (A) n = 3, or (B) silica ([−] n = 12, YVAD n = 4, ZVAD n = 6, K777 n = 8, Ca074Me n = 3), LLOMe ([−] n = 5, ZVAD n = 2, K777 n = 4), CCs ([−] n = 5, ZVAD n = 3, K777 n = 3). Statistical analysis was performed by 1‐way ANOVA and Dunnett's multiple comparisons test (A), or 2‐way ANOVA and Dunnett's multiple comparisons test (B). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.