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. 2017 Oct 11;102(6):1371–1380. doi: 10.1189/jlb.1A0816-371R

Figure 4.

Figure 4

In vivo disruption of Trim9 function in Mϕs significantly alters cell shape.

(A) Zebrafish Mϕs expressing Tg (mpeg1.1:EGFP), Tg (mpeg1.1:Trim9,EGFP), or Tg (mpeg1.1:∆RINGTrim9,EGFP) on the Tg (mpeg1.1:mCherry) background were photographed to assess cell shape. Cells from each transgenic background were photographed for quantifying circularity. Scale bar, 20 μm. (B) Mϕs from Tg (mpeg1.1:mCherry) larvae injected with Tg (mpeg1.1:∆RINGTrim9,EGFP), but not expressing ∆RINGTrim9 (e.g., EGFP) were photographed to assess cell shape. Scale bar, 20 μm. (C) Circularity scores are shown for individual cells. Mϕs that are mCherry‐positive, but EGFP‐negative were analyzed from Tg (mpeg1.1:∆RINGTrim9,EGFP) mosaic larvae including those displayed in (B). Mϕs that are mCherry‐positive and EGFP‐positive were analyzed from Tg (mpeg1.1:EGFP), Tg (mpeg1.1:Trim9,EGFP) and Tg (mpeg1.1:∆RINGTrim9,EGFP) mosaic larvae including those displayed in (A). Scale: 0–1, where 1 indicates a perfect circle. Data are presented as box‐and‐whisker plots (n = 17). *P < 0.05.