Figure 6.

Adoptive transfer of ADAM23 knockdown DCs results in impaired T cell response in vivo. OT‐II CD4⁺ T cells were harvested and labeled with CFSE before intravenous injection into a WT mouse. Subsequently, ADAM23 knockdown or control mDCs pulsed with OVA_323–339 peptide were injected into the same WT mouse. The CFSE‐labeled, T cell‐only group served as internal controls. After 3–5 d, mice were euthanized, and spleen and lymph nodes were collected. Cells were stained for IFN‐γ, IL‐4, and CD4 before flow cytometric analysis. Isotype controls were used to establish gating strategies. All CD4⁺CFSE⁺ cells were gated and displayed in the dot plot; nonlabeled CFSE subsets were excluded (by gating out). (Upper) Dot plots show CFSE versus IFN‐γ expression in T cells from the following groups: T cells only (i.e., no antigen‐bearing DC added), control DC (siControl) pulsed with antigen, and ADAM23 knockdown DC (siADAM23) pulsed with antigen. (Lower) CFSE versus IL‐4. Bar graphs represent that of quadruplicates per group; the study is representative of 2 independent experiments. **P < 0.01.