Figure 8.

Neutralization of α(v)β(3) restrains T cell proliferation and IL‐2 production. Neutralizing antibodies to α(v)/CD51 or β(3)/CD61 were added at the point of OVA_323–339‐pulsed mDCs culturing with naïve CD4⁺ T cells. Additionally, respective antibodies were added again at 6 h into the DC–T cell coculture. Isotype‐matched antibodies were used as internal controls. (A) For proliferation, T cells were prelabeled with 2.5 µM CFSE before culture with DCs in the presence of neutralizing or isotype control antibodies. After 72 h, T cells were harvested and evaluated for proliferation using flow cytometric analyses. Dashed lines represent unstimulated, CFSE‐labeled T cells as nondividing control. Filled histograms are CFSE‐labeled T cells primed by respective DC populations in the presence of respective neutralizing antibodies or isotype controls. Bar graph depicts mean percentage of proliferating CD4⁺ T cells in 3 independent experiments. (B) Supernatant was collected at the 72 h time point and evaluated for production IL‐2; data are representative of 3 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.