Skip to main content
. 2016 Jan 11;100(1):177–184. doi: 10.1189/jlb.3AB1115-507R

Figure 5.

Figure 5

Inhibition of PTK and Rac GTPase activation abrogates TLT2 translocation to the cell surface. Neutrophils were pretreated with genistein (100 μM) and then activated with fMLP (fMLF; 1 μM) or fMLP + Cyto B (5 μM) for 30 min at 37°C to induce degranulation. Flow cytometric analysis was used to monitor the fold increase in expression for (A) CD11b, (B) CD63, and (C) TLT2. The data are depicted as means ± sd of the fold increase in MFI. Data are from healthy donors, n = 5. *P < 0.05, **P < 0.01. (D) Neutrophils were pretreated with various doses of the Rac inhibitor NSC23766 and activated with fMLP (1 μM) or fMLP + Cyto B (5 μM) for 30 min at 37°C to induce degranulation. Surface expression of CD11b, CD63, and TLT2 was measured by flow cytometry. The means ± sd of the fold increase in MFI are depicted. Data are from healthy donors, n = 3. *P < 0.05.