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. 2014 Mar 19;34(12):4303–4308. doi: 10.1523/JNEUROSCI.3111-13.2014

Figure 2.

Figure 2.

Specific identity of Fezf2+CTB+ IT-PNs in L5 of mature M1. A, We targeted identified Fezf2+/− CTB+ IT-PNs with patch-clamp recordings. Dashed lines indicate the recording electrode position on a GFP− neuron. B, Single-cell RT-PCR products for Fezf2 mRNA from four GFP+CTB+, four GFP−CTB+, and one wild-type (WT) IT-PNs. Amplification used two primer sets, either 1 or 2, to generate product lengths of 118 or 406 bp, respectively. C, Two broad groups of L5 IT-PNs were segregated in an unsupervised cluster analysis, calculated using 19 parameters measured for each Fezf2+CTB+, Fezf2−CTB+, or wild-typeCTB+ IT-PN. D, Pial depth position (as a fraction of cortical thickness) identified L5A neurons in C (A in Group 2). NTC, No template control; RT−, GFP+ single-cell sample without reverse transcription; +con, positive control; M, marker; WM, white matter.