Table 1.
Overview of the antibodies used in the study, including the primary antibodies, the corresponding control peptides, as well the F(abI)2 and fluorescent antibodies
| Antigen | Control peptide | Primary antibody | Antibody species | F(abI)2 antibody | F(abI)2 species | Fluorophore [fluorphore species] |
|---|---|---|---|---|---|---|
| PV | Abcam ab45541 | Swant PV 235 | M(a)PV | Jackson ImmunoResearch 315-006-003 | R(a)M | Molecular Probes Invitrogen A-31570a [D(a)M], A-31572a [D(a)R], A-21432a [D(a)G] |
| CB | Abcam ab60452 | Swant CB 38 | R(a)CB | Jackson ImmunoResearch 111-006-003 | G(a)R | |
| CR | Abcam ab73479 | Chemicon AB5054 | R(a)CR | |||
| GluA1 | Abcam ab28424 | Chemicon AB1504 | R(a)GluA1 | — | — | Molecular Probes Invitrogen A-21206b [D(a)R], A-21202b [D(a)M] |
| GluA2 | Abcam ab25708 | Chemicon MAB397 | M(a)GluA2 | — | — | |
| GluA3 | Abcam ab132969 | Chemicon MAB5416 | M(a)GluA3 | — | — | |
| GluA4 | Abcam ab152441 | Upstate 06-308 | R(a)GluA4 | — | — |
Untagged F(abI)2 antibodies were used to block same-species primary antibodies. The F(abI)2 and fluorescent antibodies used depended on the primary antibody species, as described in Materials and Methods. The species are indicated in capital letters, with M for mouse, R for rabbit, G for goat, and D for donkey.
aAlexaFluor 555.
bAlexaFluor 488.