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Infiltrating peripherally derived macrophages are more susceptible to apoptosis than microglia after SCI. A–C, Single-plane confocal images showing TUNEL, Iba1, EGFP, and DAPI labeling at 3, 5, and 7 d after SCI. Ai–Ci, Show DAPI-positive nuclei. Aii–Cii, Show TUNEL-positive nuclei. Aiii–Ciii, Merged Iba1, EGFP, and TUNEL. Note that the TUNEL-positive apoptotic cells (arrows) are also EGFP+ (Aiii–Ciii), indicating that they are peripherally derived macrophages. Scale bar, 10 μm.
