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The effect of ATP requires DGK. A, Patch from rdgA;ninaE;trpl flies, lacking the DGK (rdgA) and rhodopsin (ninaE) and expressing TRP was silent, but was activated by DAG and could not be shut off by ATP (n = 6). B, Addition of ATP to a trpl patch in the presence of the DGK inhibitor R59022 did not affect the high TRP activity (n = 7).
