Figure 7.

Light increments unsaturated DAGs but not PUFAs in rhabdomeral membranes. A, Total lipid separation from a membrane fraction obtained by means of UPLC-MS (see Materials and Methods) from eyes pre-exposed to darkness (top) and illumination (bottom). TIC of mass spectra recorded in positive ion mode. B, Quantification of DAG species in light and dark conditions. C–H, Quantification of unsaturated DAGs, in further detail; *p < 0.05, **p < 0.01. I, TIC recorded in negative ion mode. J, Quantitative analysis of total free fatty acids. K–M, Quantitative analysis of PUFAs in further detail. There were no statistical differences in PUFAs (p > 0.05). Mean ± SEM (n = 3).