Figure 6.

SPN specifically regulated the synaptic accumulation of GluN2B receptors. a, b, The protein contents of NMDA receptor GluN1, GluN2B, and GluN2A subunits at synaptosomal membrane fraction (P3; a) or homogenates (b) were examined at day 3 after intrathecal injection of recombinant adenovirus encoding GFP, SPN(F451A), and SPN(WT) in rats. Equal protein loadings were indicated by β-actin signals. The graph summarized the percentage changes of NMDAR subunits. n = 6 experiments for each subunit. *p < 0.05 relative to GFP control. c, Effects of GluN2B-selective antagonist ifenprodil on NMDAR-EPSC amplitudes. The horizontal bar indicated the period of extracellular ifenprodil perfusion. n = 6 neurons in each group.