Figure 3.

Acute inhibition of cGKs decreases the proportion of nerve terminals that undergo strong unloading. A, Experimental protocol. B, Distribution of strong or weak unloading boutons from control cells and those exposed to KT5823 (1 μm) for 30 min before the loading step and during the washing period. C, Mean traces of strong FM1-43 unloading boutons from control cells (N = 1158, n = 5 coverslips from 3 different cell cultures) and those exposed to KT5823 (1 μm) for 30 min (N = 263, n = 5 coverslips from 2 cell cultures). D, Mean traces of weak FM1-43 unloading boutons from control cells (N = 902, n = 5 coverslips from 3 different cell cultures) and those exposed to KT5823 (1 μm) for 30 min (N = 1060, n = 5 coverslips from 2 cell cultures).