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. 2014 Jun 25;34(26):8788–8799. doi: 10.1523/JNEUROSCI.0103-14.2014

Figure 4.

Figure 4.

cGKII inhibition after the loading step does not impair SV recycling. A, Experimental protocol showing the two unloading stimuli. B, Mean traces of FM1-43 unloading boutons from control cells (N = 3668 from n = 7 coverslips from 3 different cultures), cells exposed to KT5823 (1 μm, 30 min) after FM1-43 loading (N = 1382 from n = 4 coverslips from 3 different cultures) and cells exposed to KT5823 (1 μm, 30 min) before FM1-43 loading (N = 1686 from n = 5 coverslips from 3 different cultures). C, Cumulative probability plots of unloading after the first stimulus (10 s, 50 mm KCl + 1 min, 5 mm KCl) for control cells or cells incubated with KT5823 before (p = 0.000, Kolmogorov–Smirnov) or after (p = 0.155, Kolmogorov–Smirnov) loading. D, Cumulative probability of unloading after the second stimulus (2 min, 50 mm KCl) in control cells or cells incubated with KT5823 before (p = 0.000, Kolmogorov–Smirnov) or after (p = 0.000, Kolmogorov–Smirnov) loading.