Figure 6.
The recycling and resting pool sizes are modified by cGK inhibition. A, Mean traces of 50 mm KCl (50 s) elicited vGlut1-pHluorin fluorescence in the presence of bafilomycin normalized to 50 mm NH4Cl-elicited fluorescence (maximal fluorescence) in control (n = 11 coverslips from 4 different cultures) and KT5823-treated neurons (n = 6 coverslips from 4 different cultures). Inset, Kinetics of the KCl-elicited fluorescence increase in the presence of bafilomycin, indicating the τ value (time to reach half-maximal fluorescence). B, Mean traces of vGlut1-pHluorin fluorescence in the presence of bafilomycin (absolute values, a.u.) in control (n = 11 coverslips from 4 different cultures) and KT5823-treated neurons (n = 6 coverslips from 4 different cultures) showing the membrane fluorescence before stimulation, as well as the increase elicited by 50 mm KCl (50 s) and NH4Cl perfusion.