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. 2014 Jun 25;34(26):8788–8799. doi: 10.1523/JNEUROSCI.0103-14.2014

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Copyright © 2014 the authors 0270-6474/14/348788-12$15.00/0

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Figure 9. — Heterogeneous profiles for vGlut1-pHluorin fluorescence recovery after strong stimulation (15 s, 50 mm KCl) in control cells and those exposed to KT5823 for 24 h. A, Mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence for the total number of boutons analyzed in control (N = 646 boutons, n = 10 coverslips from 4 different cultures) and KT5823-treated neurons (N = 362 boutons, n = 8 coverslips from 4 different cultures). Inset: Normalized fluorescence recovery adjusted to monoexponential decay, τ (time constant for decay in seconds). B, Percentages of the five types of profiles found in control (n = 10 coverslips) and KT-treated cells (n = 8 coverslips). C, Profile 1: mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence in control (N = 214 boutons) and KT5823-treated neurons (N = 165 boutons). D, Profile 2: mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence in control (N = 180 boutons) and KT5823-treated neurons (N = 62 boutons). E, Profile 3: mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence in control (N = 128 boutons) and KT5823-treated neurons (N = 46 boutons). F, Profile 4: mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence in control (N = 57 boutons) and KT5823-treated neurons (N = 56 boutons). G, Profile 5: mean traces of KCl-elicited vGlut1-pHluorin fluorescence normalized to NH₄Cl-elicited fluorescence in control (N = 67 boutons) and KT5823-treated neurons (N = 33 boutons).