Table 1.

Time constants for the exocytosis and endocytosis associated with the different response profiles after strong and sustained stimulation

Profiles	Time to reach half-maximal response (τ, s)			Time recovery constants τ1 and τ2 (s)
	Control	KT5823	p	Control	KT5823	p
1	6.41 ± 0.10	5.99 ± 0.41	>0.05	13.50 ± 1.71	10.24 ± 0.69	0.113492
				9.32 ± 0.76	19.71 ± 4.34*	<0.00001
2	6.06 ± 0.05	6.27 ± 0.13	>0.05	35.39 ± 1.41	37.62 ± 1.38	0.261021
3	6.73 ± 0.20	5.92 ± 0.18	>0.05	14.57 ± 0.46	19.07 ± 0.72*	<0.00001
4	4.93 ± 0.10	4.50 ± 0.19	>0.05	32.91 ± 6.62	37.77 ± 6.59	0.610233

Cells were stimulated with 50 mm KCl for 50 s and the increase in fluorescence was adjusted to (F) = F_max*t/(τ + t), where τ is the time necessary to reach half-maximal fluorescence. The fluorescence decay was adjusted to F = F₀+Ae^−t/τ, where τ is the time constant for the basal recovery. Data are mean ± SEM from individual experiments control (control, n = 10; KT-5823-treated cells, n = 8).

* represents the significance between the control and the KT5823-treated cells.